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Izon Science Support Centre

Particle Measurement

Particle Measurement

Get expert tips on how to get the best data out of your particle measurement system.

Technical Documentation

TRPS Reagent Kit and TRPS Training Kit documents

TRPS Reagent Kit Specifications and Preparations Guide

Reagent Kit: Safety Data Sheet (SDS)

Calibration Particles: Safety Data Sheet (SDS)

Training

I'm new to TRPS, where do I start?

What is in the TRPS training kit?

What is TRPS?

How does TRPS work?

Which way are the particles travelling?

What do each of the tunable parameters do?

What are the parameters to look out for when performing a TRPS measurement?

What forces act on the nanoparticles?

What is zeta potential and how does TRPS measure it?

Why measure zeta potential with TRPS instead of Zetasizer?

How does the instrument measure particle concentration?

Why do I have to perform multi-pressure measurements?

Concentration fraction: what is it and why should I use it?

How does the instrument measure particle diameter?

Pipetting Techniques

What do I need to consider when measuring the zeta potential of biological samples?

Fundamentals to Zeta Potential Measurement with TRPS

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Calibration

The importance of calibration recordings with TRPS

What are Izon calibrated particles and how should they be stored?

How do I prepare the calibration particles?

How often should I prepare fresh calibration solutions?

Can the calibration particle be used as supplied or do they require dilution?

What dilution factor should I use for the calibration particles?

How do I calculate a dilution factor?

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Sample Preparation

What is the size analysis range of the instrument?

Does my sample need to be a charged particle?

What sample volume is required?

How much should I dilute my sample if the starting concentration is unknown?

How do I avoid contamination?

What electrolyte can be used for TRPS measurements?

What factors do I need to consider when choosing an electrolyte?

Does the pH value of electrolyte matter when completing measurements on Izon TRPS systems?

Overview: Measurement of particles on larger nanpores (NP1000 and larger)

What are the recommended protocols for measuring virus samples?

What are the recommended EV isolation/purification methods for TRPS measurements?

How do I prepare qEV fractions for TRPS analysis?

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Nanopores

Nanopore characterisation and set-up overview

Choosing a nanopore suited to your sample

How is the size of the nanopore determined?

What is nanopore coating procedure and why is it important?

Why do I need to re-equilibrate the nanopore with electrolyte after the coating procedure?

What is the best practice for cleaning the nanopore?

How long can I use a Nanopore?

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Troubleshooting

How can I improve my TRPS measurement?

How do I deal with a partial nanopore blockage during a recording?

My sample continually blocks the pore. What can I do to prevent this?

What causes bubbles in the lower fluid cell?

How do I get rid of air bubbles in the lower fluid cell?

How do I manage fluid flow rate for larger nanopores (NP1000 and larger)?

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TRPS Fundamentals

When using the Exoid, why does the sample measurement current need to be within 10% of the calibration measurement current?