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Particle Measurement
Access support resources and workflows to help you get the most from TRPS measurements.
Training
- I'm new to TRPS, where do I start?
- What is in the TRPS training kit?
- What is TRPS?
- How does TRPS work?
- Which way are the particles travelling?
- What do each of the tunable parameters do?
- What are the parameters to look out for when performing a TRPS measurement?
- What forces act on the nanoparticles?
- What is zeta potential and how does TRPS measure it?
- Why measure zeta potential with TRPS instead of Zetasizer?
- How does the instrument measure particle concentration?
- Why do I have to perform multi-pressure measurements?
- Concentration fraction: what is it and why should I use it?
- How does the instrument measure particle diameter?
- Pipetting Techniques
- What do I need to consider when measuring the zeta potential of biological samples?
Calibration
- The importance of calibration recordings with TRPS
- What are Izon calibrated particles and how should they be stored?
- How do I prepare the calibration particles?
- How often should I prepare fresh calibration solutions?
- Can the calibration particle be used as supplied or do they require dilution?
- What dilution factor should I use for the calibration particles?
- How do I calculate a dilution factor?
Sample Preparation
- Does my sample need to be a charged particle?
- What sample volume is required?
- How much should I dilute my sample if the starting concentration is unknown?
- How do I avoid contamination?
- What electrolyte can be used for TRPS measurements?
- What factors do I need to consider when choosing an electrolyte?
- Does the pH value of electrolyte matter when completing measurements on Izon TRPS systems?
- Overview: Measurement of particles on larger nanpores (NP1000 and larger)
- What are the recommended protocols for measuring virus samples?
- What are the recommended EV isolation/purification methods for TRPS measurements?
- How do I prepare qEV fractions for TRPS analysis?
Nanopores
- Nanopore characterisation and set-up overview
- Choosing a nanopore suited to your sample
- How is the size of the nanopore determined?
- What is nanopore coating and why is it important?
- Why do I need to re-equilibrate the nanopore with electrolyte after the coating procedure?
- What is the best practice for cleaning the nanopore?
- How long can I use a Nanopore?
Troubleshooting
- How can I improve my TRPS measurement?
- How do I deal with a partial nanopore blockage during a recording?
- My sample continually blocks the pore. What can I do to prevent this?
- What causes bubbles in the lower fluid cell?
- How do I get rid of air bubbles in the lower fluid cell?
- How do I manage fluid flow rate for larger nanopores (NP1000 and larger)?