What is the best practice for cleaning the nanopore?

Removal of the nanopore with electrolyte and salts within it will shorten the lifespan of a nanopore. Salt crystals will form within the pore often rendering it impossible to wet or with reduced resolving capability when next used.

At the end of an experiment it is critical to wash out the pore before removal of the nanopore from the qNano instrument. 

Nanopore washout solution
Mix 100mL of deionised water and 100 µL of Wetting Solution Concentrate (supplied in Reagent Kit)


Clean the Nanopore

  1. Remove applied pressure.
  2. Stretch the nanopore to 47.5mm.
  3. Remove the upper fluid cell. A droplet of fluid should remain on top of the nanopore. Immediately pipette 35 µL of Measurement Electrolyte to the centre of the nanopore surface to maintain pore hydration.
  4. Rinse the upper fluid well with deionized water and dry with compressed gas or lint-free tissues.
  5. Replace the content in the lower fluid well with Measurement Electrolyte.
  6. Gently wipe the top of nanopore with lint-free tissue.
  7. Immediately fit the upper fluid cell and pipette 35 μL of the Measurement Electrolyte into the upper fluid well ensuring no bubbles present.
  8. Apply maximum pressure for approximately 5 minutes.
  9. Once completed, repeat Step 3-8 with filtered Nanopore Washout Solution. During fluid changeover, add an aliquot of the washout solution to the top of nanopore to avoid drying.
  10. Once completed, remove the fluid from the lower fluid well.
  11. Remove the nanopore from the instrument, rinse with deionized water and dry with compressed gas/lint-free tissue.
  12. Store in a clean and closed container/bag.

    Thorough cleaning and proper handling of the nanopore is paramount in increasing the probability of successful re-use of the nanopore. It is good practice to keep a record of the type of sample that a nanopore has been exposed to. 

     

    Some biological samples, such as plasma, contain compounds that can stick to the nanopore. Ethanol may aid in cleaning such nanopores. Nanopores that have been exposed to virus samples will also need to be decontaminated with 70% ethanol.

     

    We recommend that nanopores that have been exposed to biological samples be discarded after use to minimise the risk of contamination and to avoid degradation of the nanopore performance.