What dilution factor should I use for the calibration particles?

The table below states target concentrations for the sample and calibration particles depending on the selected nanopore.

The concentration of the calibration particles is written on the vial label.

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  • When working at high stretch (>47 mm), it may be better to select the next size up calibration particles.
  • For most calibration particles, a 1:1000 dilution is suitable. A dilution factor of 1:500 is suitable for CPC200. 
  • If the calibration particle rate is too low, make a more concentrated solution.
  • Neutral calibration particles are available upon request. This is relevant for any zeta potential work or for measurements using a negative voltage. 

 

Contact Izon Support for guidance on optimising operating conditions.