What electrolyte can be used for TRPS measurments?

An electrolyte is used to suspend the sample and calibration particles. The same electrolyte is also used to clean the fluid cell and nanopore in between sample and calibration measurements.
When selecting an electrolyte, the user should consider how their particles may behave in this electrolyte, particularly if the chosen electrolyte differs from that used in alternative sample measurements and analyses. For example, some particles will aggregate more readily in higher salt concentrations. Check with Izon (support@izon.com) if you intend to use a non-standard electrolyte as certain chemicals may cause damage to the fluid cell and/or nanopores.

Izon recommends using an electrolyte with a molarity >0.02 M. Typically, an electrolyte with a salt concentration of ~0.1 M (e.g. PBS) is used. However, the user is able to use alternative electrolytes and Izon Science has done so in many applications. Operating with an electrolyte <0.02 M will result in a very small current and associated blockade magnitudes and potentially, blockades (particles) will go undetected. 


A large variety of electrolytes have been used with Izon instruments e.g. PBS, cell culture media, KCl. Izon regularly use PBS based electrolyte. Below is a summary of recommended salt concentration for each nanopore type:

salt concentration table


* For detection of smaller particle sizes (<100nm), high molarity electrolyte can improve blockade signal. We recommend using an electrolyte which has a conductivity equivalent to 2x PBS, minimum. Note that less stable particles tend to aggregate in higher molarity electrolyte.


Surfactants can improve surface wetting and reduce aggregation. The addition of surfactant aids in establishing a stable baseline current.