Izon recommends using an electrolyte with a molarity >0.02 M. Typically, an electrolyte with a salt concentration of ~0.1 M (e.g. PBS) is used. However, the user is able to use alternative electrolytes and Izon Science has done so in many applications. Operating with an electrolyte <0.02 M will result in a very small current and associated blockade magnitudes and potentially, blockades (particles) will go undetected.
A large variety of electrolytes have been used with Izon instruments e.g. PBS, cell culture media, KCl. Izon regularly use PBS based electrolyte. Below is a summary of recommended salt concentration for each nanopore type:
* For detection of smaller particle sizes (<100nm), high molarity electrolyte can improve blockade signal. We recommend using an electrolyte which has a conductivity equivalent to 2x PBS, minimum. Note that less stable particles tend to aggregate in higher molarity electrolyte.
Surfactants can improve surface wetting and reduce aggregation. The addition of surfactant aids in establishing a stable baseline current.