What are the recommended EV isolation/purification methods for TRPS measurements?

All biological samples are complex and the user must optimise the protocols for their own biological particles systems. Some raw samples cannot be directly run on a qEV and analysed with TRPS without further preparation steps, such as sequential centrifugation or concentration steps.

For some biofluids with lower levels of EVs and proteins, the sample can be concentrated before application to qEV columns to enable larger volumes to be processed (not applicable to serum and plasma that have very high levels of protein and cannot be readily concentrated). The exact protocol may require modification depending on the sample, its viscosity and other preparation steps. 

Izon provides example protocols for preparation of extracellular vesicles from common sample types: