How is the size of the nanopore determined?

Each nanopore is characterized by measuring a calibration particle set to calculate the hole size.

A calibration particle of a known size is measured on a known stretch through an unknown sized hole. Because the size of the blockade caused by the particle is a direct relationship with the size of the hole then using calculations we can determine the Mid Range Particle (MRP) of each individual nanopore. Each nanopore category has an acceptable MRP range which allows for the resolution of the particle sizes recommended. For example, an NP100 would have an MRP value of 90-115nm.