- Izon Science Support Centre
- TRPS Measurement
- Sample Preparation and Measurement
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TRPS Measurement
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The Exoid
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The Exoid Control Suite Software
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qNano
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qNano Control Suite Software
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Data Analysis
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TRPS Consumables
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qEV Columns
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Automatic Fraction Collector (AFC) V2
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Automatic Fraction Collector (AFC) V1
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General
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qEV Concentration Kits
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qEV RNA Extraction kits
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Safety Data Sheets (SDS)
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qEV Kits
Does the pH value of electrolyte matter when completing measurements on Izon TRPS systems?
Yes. The qNano/Exoid system components and consumables which includes fluid cells, calibration particles, and nanopores are compatible with electrolytes within a pH range of 4-10. Ideally electrolytes with a pH of 7-7.4 (physiological conditions) are used. If the electrolyte that is used is of a different pH, please contact Izon to enquire about material compatibility testing.
More importantly, Izon calibration particles are made of carboxylated polystyrene which have a pKa of approximately 5. These calibration particles are stable in electrolyte with pH of 7-7.4. If the electrolyte solution that is used has a pH of 5.5 (for example), this will cause aggregation of the calibration particles as they will carry minimal charge at pH 5.5.