- Izon Science Support Centre
- TRPS Measurement
- Troubleshooting: Blockages and Bubbles
What causes bubbles in the lower fluid cell?
Bubbles in the lower fluid cell can come from a number of sources:
- If the lower fluid cell does not have sufficient liquid in it, an air pocket may become trapped in the gap between the fluid and the nanopore.
- Incorrect pipetting technique may introduce air bubbles into the lower fluid cell.
- Depending on the environmental conditions, evaporation of fluid from the lower fluid cell may cause an air pocket. This usually only happens after a few hours of operation.
- Air is trapped in the porous electrode paste (in the lower fluid cell) and can come out when fluid is loaded. Pre-wetting the lower fluid cell with electrolyte containing surfactant helps to alleviate bubble formation in the bottom well during the course of a measurement.