What are the recommended protocols for measuring virus samples?

Virus samples are inherently unstable in the fluid-phase and will form aggregates. 

 

Below are some recommendations for virus preparation for measurements on the qNano: 

  • Centrifuge the virus sample. High speed if possible, 10,000 g for 10 minutes. If not possible, centrifuge at low speed, 3000 g for 10 minutes. 
  • Filter preferably through a 0.22 micron filter. If this is not possible, filter through a larger filter mesh. 
  • For raw viruses, start with a 1:100 or 1:500 dilution into a clean filtered electrolyte. Adjust dilution as necessary.
  • Use a new nanopore whenever possible. Use of larger nanopore may be necessary to quantify the aggregates.

 

As per all measurement on the smaller nanopores, use of Izon Reagent Kit is necessary to assist with measurement stability.