The average working time from a nanopore is about 8 to 10 hours.
However, the actual size of the aperture itself can drift over time, mainly due to the following factors:
- Our apertures are prone to gradual drifts in size, as the thermoplastic absorbs water after a few hours of hydration. This is not preventable for long measurement sequences, and so we recommend to run calibrations every half hour during long measurement plans to account for this.
- The extra cleaning and unblocking steps required to resolve the issues due to unfiltered reagent may increase the frequency and intensity of mechanical stresses experienced by the aperture, and this in turn often leads to more rapid and more significant size increases.
- Non-specific binding of biological macro molecules to the aperture walls can cause changes in nanopore size, as well as blockages and instability.
Most causes of instability (and by association, drifts in pore size) can be mitigated or eliminated by using the Izon reagent kit, for the following reasons:
- The single-use filters help prevent blockages by excluding large contaminant particles from solutions
- Non-specific binding of biological matter to the pore can be prevented by coating the pore using the Izon coating solution before running biological samples, to create a protective chemical barrier.
- The Izon wetting solution concentrate is a surfactant solution that also helps to wet nanopores more effectively. Adding a surfactant also reduce the rate of particle aggregation for non-biological samples.
Variations of all of these components could be researched and purchased separately in different combinations, but can purchase the Reagent Kit as a complete solution for sample preparation that will save a lot of time and labour during method development, as we have found the optimal concentrations to use for each reagent, to maximise pore stability.