What is nanopore coating and why is it important?

Izon has developed a coating procedure to reduce the impact of nanopore surface modification, which can occur as a result of interaction with biological samples. The coating procedure uses Izon Coating Solution (ICS), which treats the nanopore and minimises non-specific binding of particles to the pore material. This step may be helpful in the following situations:

• If the sample has not been purified prior to analysis (e.g. pure plasma or pure cell culture medium)
• If initial measurements are proving problematic
• If it is difficult to remove all particles when cleaning the pore

For effective coating, ICS must have not expired and must have been stored appropriately. Beyond the recommended storage time, the reagent will deteriorate and cause instability problems.