- Izon Science Support Centre
- TRPS Measurement
- Sample Preparation and Measurement
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TRPS Measurement
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The Exoid
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The Exoid Control Suite Software
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qNano
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qNano Control Suite Software
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Data Analysis
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TRPS Consumables
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qEV Columns
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Automatic Fraction Collector (AFC) V2
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Automatic Fraction Collector (AFC) V1
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General
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qEV Concentration Kits
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qEV RNA Extraction kits
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Safety Data Sheets (SDS)
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qEV Kits
Why do I need to re-equilibrate the nanopore with electrolyte after the coating procedure?
The coating solution is viscous compared to the electrolyte. Follow the steps below to complete the equilibration procedure:
- Remove the upper fluid cell. During fluid changeovers, to maintain pore hydration it is best to add a drop of measurement electrolyte to the top of nanopore surface. Do not wipe dry the top of nanopore surface at this stage.
- Wash out the upper fluid cell with DI water and dry with compressed gas.
- Rinse out the lower fluid cell with DI water 2-3 times and dry. Once completed, load the lower fluid cell with electrolyte.
- Wipe the top of the nanopore surface, immediately replace the upper cell and load electrolyte into the upper fluid cell.
- Connect the pressure nozzle and apply a maximum pressure for 10-15 min to flush out the high viscosity concentrated ICS from the pore.