- Izon Science Support Centre
- qEV Columns
- Product Overview: qEV Size Exclusion Columns
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TRPS Measurement
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The Exoid
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The Exoid Control Suite Software
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qNano
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qNano Control Suite Software
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Data Analysis
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TRPS Consumables
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qEV Columns
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Automatic Fraction Collector (AFC) V2
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Automatic Fraction Collector (AFC) V1
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General
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qEV Concentration Kits
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qEV RNA Extraction kits
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Safety Data Sheets (SDS)
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qEV Kits
How do I prepare qEV fractions for TRPS analysis?
Below are some recommendation for TRPS analysis of the qEV fractions:
- Izon Science recommend the use of the Izon Reagent Kit, which is used to precoat nanopores and used throughout the measurement process.
- Dilute the eluted fractions by 1:5 or 1:10 in Measurement Electrolyte. It is essential to filter all reagents used in preparing and running samples on TRPS to avoid contamination/particulates that can occur over time and interfere with TRPS analysis.
- Optimise the dilution to achieve a rate at the highest operating pressure of approximately 500 to 1500 particles/minute to avoid pore blockage. If the vesicle concentration is too low for TRPS analysis, you may need to concentrate the sample.