The below reference table details the range of Izon nanopores.
Analysis range is indicative of particle sizes that can be analysed across the stretch range of the nanopore. If working at:
- Lower size range, a higher concentration electrolyte than standard PBS may be used for the instrument to detect 40 nm particles.
- Higher size range, a lower concentration electrolyte than standard PBS may be used for the instrument to detect up to 11,000 nm size particles without railing the electronics.
Tips and recommendations
- If a measurement is completed via Classic Capture, voltage should be optimised when a baseline current has been established - typically to achieve a current of 120 - 140 nA in the High Gain default setting.
- For large nanopores, better results may be obtained by using a less conductive electrolyte OR switching to the Low Gain stage (via Settings). This will allow a larger voltage to be applied.
- When working at high stretch, it may be better to select larger calibration particles.
- It is possible to work below and above the suggested stretch range of 44 - 47 mm. At lower stretches there is a risk that the fluid seal to the upper fluid cell may be lost. At higher stretches there is a risk of the nanopores rupturing to a much larger size.
If your sample is very polydispersed, you may need to use a few nanopore sizes to cover the size range. For example, if your sample ranges from 100 nm to 5000 nm, you will need to use more than one membrane to detect particles across the entire size range. Shown below is a microbubble sample measured with different membranes and stretch settings.