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Izon Science Support Centre

TRPS Measurement

TRPS Measurement

Get expert tips on how to get the best data out of your TRPS measurement system.

TRPS Fundamentals

What is TRPS?

How does TRPS work?

What do each of the tunable parameters do?

The importance of calibration recordings with TRPS

Nanopore characterisation and set-up overview

Pipetting Techniques

Why measure zeta potential with TRPS instead of Zetasizer?

What do I need to consider when measuring the zeta potential of biological samples?

What is zeta potential and how does TRPS measure it?

How does the instrument measure particle concentration?

How does the instrument measure particle diameter?

What forces act on the nanoparticles?

Fundamentals to Zeta Potential Measurement with TRPS

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Troubleshooting: Blockages and Bubbles

How do I deal with a partial nanopore blockage during a recording?

What causes bubbles in the lower fluid cell?

How do I get rid of air bubbles in the lower fluid cell?

My sample continually blocks the pore. What can I do to prevent this?

Sample Preparation and Measurement

What is the Nanopore Wetting Procedure for the qNano and why is it important?

What dilution factor should I use for the calibration particles?

What is the size analysis range of the instrument?

What are the parameters to look out for when performing a TRPS measurement?

Why do I need to re-equilibrate the nanopore with electrolyte after the coating procedure?

What is nanopore coating procedure and why is it important?

How do I avoid contamination?

What are the recommended protocols for measuring virus samples?

Overview: Measurement of particles on larger nanpores (NP1000 and larger)

How do I manage fluid flow rate for larger nanopores (NP1000 and larger)?

Concentration fraction: what is it and why should I use it?

Why do I have to perform multi-pressure measurements?

Which way are the particles travelling?

How do I prepare the calibration particles?

What factors do I need to consider when choosing an electrolyte?

What electrolyte can be used for TRPS measurements?

How can I improve my TRPS measurement?

How long can I use a Nanopore?

Does the pH value of electrolyte matter when completing measurements on Izon TRPS systems?

How much should I dilute my sample if the starting concentration is unknown?

Does my sample need to be a charged particle?

What sample volume is required?

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