qEVoriginal, qEV2, and qEV10 columns can be reused; however, they must be regenerated and/or cleaned depending on use.
The Automatic Fraction Collector allows use for up to 10 times. A warning sign will appear on screen at the 5th use to remind user that there will be five more uses left.
qEVsingle columns are designed for single use only. However if further use is anticipated after collection of the vesicle fractions they must be regenerated and/or cleaned depending on use. Single use is advisable where the vesicle fractions are to be analysed using PCR or RT-PCR. This is to avoid any risk of contamination for an incorrectly cleaned column.
The columns are preforming well if the flow rate is conserved between runs.
Izon recommends doing an initial flow rate test prior to loading any sample on the column
- Wash the columns with 0.5 M NaOH
- Re-equilibrate the columns with PBS to wash out proteins and other biologicals.
- Monitor column function by plotting the protein elution curves of the column by recording absorbance at 280 nm for each fraction.