The table below shows the cleaning steps required for each of the qEV columns:
Column | Cleaning Instructions |
qEVoriginal | 10 mL 0.5 M NaOH, followed by 20 mL buffer. |
qEVoriginal Gen 2 | 8.5 mL 0.5 M NaOH, followed by 17 mL buffer. |
qEV1 | 13.5 mL 0.5 M NaOH, followed by 27 mL buffer. |
qEV2 | 90 mL buffer, followed by 2 mL 0.5 M NaOH, followed by 90 mL buffer. |
qEV10 | 140 mL buffer, followed by 5 mL 0.5 M NaOH, followed by 140 mL buffer. |
qEV10 Gen 2 | 140 mL buffer, followed by 20 mL 0.5 M NaOH, followed by 140 mL buffer. |
20% Ethanol can also be used to store columns, however if a concentrated electrolyte such as 2x PBS is used, flush the column with DI water before introducing 20% ethanol otherwise salt will precipitate out inside the column and render the column unusable.
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Single use is advisable where the vesicle fractions are to be analysed using PCR or RT-PCR.
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Degassed buffers will help avoid air bubbles forming in the gel bed. A small number of air bubbles will have minimal effect on the column performance.
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It is recommended to use a buffer with an ionic strength of 0.15 M or greater to avoid any unwanted ionic interactions between the solute molecule and the matrix.
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To avoid clogging of column filters, it is recommended to filter or low-speed centrifuge the biological sample to remove large particulate matter.