The table below shows the cleaning steps required for each of the qEV columns:
|qEVoriginal||10 mL 0.5 M NaOH, followed by 20 mL buffer.|
|qEVoriginal Gen 2||8.5 mL 0.5 M NaOH, followed by 17 mL buffer.|
|qEV1||13.5 mL 0.5 M NaOH, followed by 27 mL buffer.|
|qEV2||90 mL buffer, followed by 2 mL 0.5 M NaOH, followed by 90 mL buffer.|
|qEV10||140 mL buffer, followed by 5 mL 0.5 M NaOH, followed by 140 mL buffer.|
|qEV10 Gen 2||140 mL buffer, followed by 20 mL 0.5 M NaOH, followed by 140 mL buffer.|
20% Ethanol can also be used to store columns, however if a concentrated electrolyte such as 2x PBS is used, flush the column with DI water before introducing 20% ethanol otherwise salt will precipitate out inside the column and render the column unusable.
Single use is advisable where the vesicle fractions are to be analysed using PCR or RT-PCR.
Degassed buffers will help avoid air bubbles forming in the gel bed. A small number of air bubbles will have minimal effect on the column performance.
It is recommended to use a buffer with an ionic strength of 0.15 M or greater to avoid any unwanted ionic interactions between the solute molecule and the matrix.
To avoid clogging of column filters, it is recommended to filter or low-speed centrifuge the biological sample to remove large particulate matter.