- Izon Science Support Centre
- qEV Columns
- Care of qEV Columns
How do I clean and store the qEV column after I've collected my EVs?
The table below shows the cleaning steps required for each of the qEV columns:
20% Ethanol can also be used to store columns, however if a concentrated electrolyte such as 2x PBS is used, flush the column with DI water before introducing 20% ethanol otherwise salt will precipitate out inside the column and render the column unusable.
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Single use is advisable where the vesicle fractions are to be analysed using PCR or RT-PCR.
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Degassed buffers will help avoid air bubbles forming in the gel bed. A small number of air bubbles will have minimal effect on the column performance.
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It is recommended to use a buffer with an ionic strength of 0.15 M or greater to avoid any unwanted ionic interactions between the solute molecule and the matrix.
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To avoid clogging of column filters, it is recommended to filter or low-speed centrifuge the biological sample to remove large particulate matter.