The signal trace appears as a yellow band or upward spikes. What does this mean?

When using the high gain electronics (default), the baseline current can reach a maximum value of ±150 nA, beyond which the electronics may saturate. When using the low gain electronics, baseline current values of up to ±1800 nA may be measured.

 

If the signal trace appears as a large yellow band or as upward spikes, this is called railing. Railing is when the electronics has been saturated by a current that is too large (either positive or negative).

Baseline current keeps railing when working with larger nanopores (NP1000  and larger)

Below is the list of causes and solutions to rectify the issue:

railing baseline table

You may decrease the nanopore stretch  to reduce the background current. However, you will need to repeat the measurement sequence of calibration and sample runs.