The signal trace appears as a yellow band or upward spikes. What does this mean?

When using the high gain electronics (default), the baseline current can reach a maximum value of ±160 nA, beyond which the electronics may saturate. When using the low gain electronics, baseline current values of up to ±1800 nA may be measured.

If the signal trace appears as a large yellow band or as upward spikes, this is called railing. Railing is when the electronics has been saturated by a current that is too large (either positive or negative).

Railing Below is the list of causes and solutions to rectify the issue:

railing baseline table

You may decrease the nanopore stretch to reduce the background current. However, you will need to repeat the measurement sequence of calibration and sample runs.