qNano & TRPS Measurement
Control Suite Software
Automatic Fraction Collector (AFC)
The signal trace appears as a yellow band or upward spikes. What does this mean?
When using the high gain electronics (default), the baseline current can reach a maximum value of ±150 nA, beyond which the electronics may saturate. When using the low gain electronics, baseline current values of up to ±1800 nA may be measured.
If the signal trace appears as a large yellow band or as upward spikes, this is called railing. Railing is when the electronics has been saturated by a current that is too large (either positive or negative).
Below is the list of causes and solutions to rectify the issue:
You may decrease the nanopore stretch to reduce the background current. However, you will need to repeat the measurement sequence of calibration and sample runs.