1. Izon Science Support Centre
  2. qNano
  3. Troubleshooting: Blockages and Bubbles

The rate plot does not display a constant gradient. Why is this important and what can be done to rectify the issue?

The rate plot is used during a recording to evaluate the quality of the data. The rate plot must maintain a linear gradient, as shown below:

If there is a change to the rate of particles passing through the nanopore then this will be reflected by a change in the gradient of the rate plot. This is demonstrated in the rate plot shown below:

The rate plot does not display a constant gradient. Why is this important  and what can be done to rectify the issue?

When this observation occurs, the blockade magnitude and particle rate measurements will be incorrect and will result in inaccurate size and concentration calculations. The system setup must be corrected:

rate gradient change table

For tips on unblocking a nanopore, read this article for advice.