- Izon Science Support Centre
- qNano
- Troubleshooting: Blockages and Bubbles
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TRPS Measurement
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The Exoid
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The Exoid Control Suite Software
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qNano
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qNano Control Suite Software
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Data Analysis
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TRPS Consumables
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qEV Columns
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Automatic Fraction Collector (AFC) V2
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Automatic Fraction Collector (AFC) V1
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General
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qEV Concentration Kits
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qEV RNA Extraction kits
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Safety Data Sheets (SDS)
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qEV Kits
The rate plot does not display a constant gradient. Why is this important and what can be done to rectify the issue?
The rate plot is used during a recording to evaluate the quality of the data. The rate plot must maintain a linear gradient, as shown below:
If there is a change to the rate of particles passing through the nanopore then this will be reflected by a change in the gradient of the rate plot. This is demonstrated in the rate plot shown below:
When this observation occurs, the blockade magnitude and particle rate measurements will be incorrect and will result in inaccurate size and concentration calculations. The system setup must be corrected:
For tips on unblocking a nanopore, read this article for advice.