TRPS provides the physical diameter of individual sample particles through measurement of the sample followed by a calibration measurement. Therefore, it is critical that both the sample and calibration recordings are measured under identical measurement settings (nanopore, voltage, stretch, pressure, current, and electrolyte) and that an Izon-approved calibration particle is always used.
The results obtained using TRPS may differ from expected if results are compared to alternative technologies. Some technologies are highly dependent on user settings, have limited resolution or different sampling windows so the user needs to be mindful when comparing data.
Below is a list of potential causes and solutions that could compromise the TRPS measured particle size:
Some sample particles may be damaged by sonication