How should I prepare the elution buffer?

All buffers should be filtered and degassed. Degassed buffers will help avoid air bubbles forming in the gel bed. A small amount of air bubbles (refer to table below) will have minimal effect on the column performance.

air bubbles table

It is recommended to use a buffer with an ionic strength of 0.15 M or greater to avoid any unwanted ionic interactions between the solute molecule and the matrix. The column comes equilibrated in filtered PBS containing 0.05% w/v sodium azide.