How are the qEV columns validated?

We use a mix of synthetic (carboxylated polystyrene and liposomes) particles and plasma samples to validate our columns. 

 

Particle are passed through the columns and we evaluate the elution profiles by monitoring the absorbance wavelength at: 

  • 600nm for the carboxylated polystyrene particles, 
  • 497nm for the liposome particles, and 
  • 280nm for the plasma sample. 

An accurate measurement of the level of protein in fractions can be obtained using a Bradford assay.