1. Izon Science Support Centre
  2. qEV Columns
  3. Product Overview: qEV Size Exclusion Columns

How does the qEV work?

Izon’s qEV Size exclusion chromatography (SEC) columns separate particles based on their size as they pass through column packed with a porous, polysaccharide resin.

As molecules enter the resin, smaller particles become trapped in the pores and their exit from the column is delayed (Fig 1C). As liquid exits the column, sequential fractions are collected. Particles will be distributed across the fractions based on their size, with the largest particles exiting the column first and the smallest particles exiting the column last.

 

Watch this quick video on how qEV isolates extracellular vesicles

 

The benefits of using the qEV columns include:

  • Fast isolation of extracellular vesicles (EVs).
  • Each column removes >95% of contaminating background proteins, lipids, solutes, cell debris, and other particulates, while maintaining the integrity and biological properties of EVs.
  • Highly enriched EV samples mean sensitivity and accuracy of downstream assays (e.g. protein profiling, RNA profiling, etc.) are improved.

Isolations are quick and thus EV samples can be collected, prepared for analysis and analysed by a qNano Gold System in less than 60 minutes.