- Izon Science Support Centre
- qEV Columns
How can I prepare my qEV column if my downstream applications are affected by Sodium Azide?
Before using a qEV column for the first time, it is recommended to flush the column with sample buffer.
qEV columns contain <0.1% sodium azide. If this can affect your downstream sample analysis, it is advised to flush the column with 2-3 column volumes of your sample buffer before use, this will reduce the sodium azide levels below 0.01 mg/mL. If you intend to use a buffer other than PBS, the column should be flushed at least 3 times with the new buffer to exchange buffers.