How can I prepare my qEV column if my downstream applications are affected by Sodium Azide or ProClin?

Before using a qEV column for the first time, it is recommended to flush the column with sample buffer. 

qEV columns contain <0.1% sodium azide or ProClin 200. To check which, please see Does my qEV column contain sodium azide or ProClin? If these can affect your downstream sample analysis, it is advised to flush the column with 2-3 column volumes of your sample buffer before use. If you intend to use a buffer other than PBS, the column should be flushed at least 3 times with the new buffer to exchange buffers.