Relative Particle Size
All uncalibrated recordings are reported in nA. The values will update to nm (or fL) upon calibration. All calibration particles are reported in nA.
Term |
Definition |
Mean | Average value of recorded particle size |
Mode | Particle size that occurs most frequently |
Maximum | Maximum particle size recorded |
Minimum | Minimum particle size recorded |
d50 | The median particle size i.e. the particle size where half of the population falls below this value |
d10 | The particle size where 10% of the population falls below this value |
d90 | The particle size where 90% of the population falls below this value |
d90/d10 | This signifies the width of the distribution which reflects the dispersity of the sample |
The diagram below summarises the d values:
Concentration
All concentrations are reported in particles/mL.
If a concentration fraction is set, the concentration is reported with a square bracket e.g.:
All measurements obtained using the qNano assistant have an associated concentration fraction, which is the specified size range over which the concentration was calculated. In the above example, the reported concentration is for samples between 70 and 280 nm. Setting a concentration fraction allows data to be compared reliably between each measurement.
If a concentration fraction is not set, the concentration is reported for all data points collected:
Term |
Definition |
Measured Concentration | Concentration of diluted sample. This is the concentration of sample aliquot loaded in the upper fluid cell. |
Raw Concentration | Concentration of original sample. This is the concentration of the raw sample i.e. before dilution. |
If the rate for multi-pressure calibration is incorrect, i.e. rate for the higher pressure is lower than the rate for lower pressure, concentration is reported as "Not Available".
Measurement Details
Term |
Definition |
Software Version | Software version that the report is viewed on |
Capture Assistant | Specifies which qNano assistant was used e.g. custom or gold. If classic capture is used, this section will print "None" |
Investigation | File name used for measurements. All recordings in the measurement plan are automatically grouped under this file name |
Sample ID | Given sample name. Report shows the sample ID that is being highlighted |
Electrolyte | Electrolyte that was used to run the measurement as entered by the user |
Dilution | Dilution factor entered for the sample |
Pressure | Pressure value used in the measurement |
Nanopore ID | Nanopore's serial number used for the measurement |
Nanopore type | Nanopore size used for the measurement |
Calibration ID(s) | Calibration ID is used to calibrate the sample. If multi-pressure calibration was performed, all associated calibration IDs are displayed here |
Notes | Any notes entered during "Measurement Setting" |