Are samples diluted when passed through the qEV columns?

Yes. The dilution factor depends on both the fraction volume and which fractions are pooled. The highest concentration can usually be obtained by measuring each fraction and using the two fractions with highest EV and lowest protein concentration. Thus there is a compromise between recovery and purity of the EVs collected.

Elution profiles for each column can be found in the user manuals. However, we recommend you determine the elution profile of your sample type and optimise the fraction volume and pooling to suit your application.